tgf β smad signaling inhibitor sb431542  (MedChemExpress)

Journal: Frontiers in Oncology

Article Title: Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway

doi: 10.3389/fonc.2025.1562976

Figure Lengend Snippet: Primer sequences for RT-qPCR.

Article Snippet: To activate or inhibit the TGFβ1/SMAD pathway, cells were treated with an agonist (TGFβ1, 10 ng/ml) or antagonist (SB525334, 5 μM) (MedChemExpress, USA).

Techniques: Sequencing

Journal: Frontiers in Oncology

Article Title: Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway

doi: 10.3389/fonc.2025.1562976

Figure Lengend Snippet: Tricellulin expressions correlated with TGFB1 and SMAD2/3 in CRC. Representative images of IHC staining demonstrating the expression of tricellulin, TGFβ1, and SMAD2/3 in normal and cancerous colorectal tissues (A) . Results of correlation analysis showing the positive correlation between tricellulin and TGFβ1 (B) and SMAD2/3 (C) in CRC tissues. Scale bar =20 μm. *** P<0.001, ****P<0.0001 .

Article Snippet: To activate or inhibit the TGFβ1/SMAD pathway, cells were treated with an agonist (TGFβ1, 10 ng/ml) or antagonist (SB525334, 5 μM) (MedChemExpress, USA).

Techniques: Immunohistochemistry, Expressing

Journal: Frontiers in Oncology

Article Title: Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway

doi: 10.3389/fonc.2025.1562976

Figure Lengend Snippet: Tricellulin regulates TGFβ/SMAD2/3 signaling pathway in CRC cells. Results of RT-qPCR showing the relative expression levels of TGFβ1, SMAD2, and SMAD3 under the indicated conditions (A, B) . Representative images (C) and quantified results (D, E) of Western blot analysis demonstrating the expression and/or activation of TGFβ1, SMAD2/3, p-SMAD2, and p-SMAD3 under the indicated conditions. n=3, * P<0.05, **P <0.01, *** P<0.001, ****P<0.0001 .

Article Snippet: To activate or inhibit the TGFβ1/SMAD pathway, cells were treated with an agonist (TGFβ1, 10 ng/ml) or antagonist (SB525334, 5 μM) (MedChemExpress, USA).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Activation Assay

Journal: Frontiers in Oncology

Article Title: Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway

doi: 10.3389/fonc.2025.1562976

Figure Lengend Snippet: Tricellulin modulates TGFβ/SMAD2/3 signaling in CRC cells. Results of RT-qPCR demonstrate the expression of tricellulin (A) , TGFβ1 (B) , SMAD2 (C) , and SMAD3 (D) in CRC cells with or without tricellulin overexpression and treated with SB525334 or vehicle. Results of RT-qPCR showing the expression of tricellulin (E) , TGFβ1 (F) , SMAD2 (G) , and SMAD3 (H) in CRC cells with or without tricellulin knockdown and treated with TGFβ1 or vehicle. Representative images (I) and quantified results of Western blot analysis demonstrating protein levels of tricellulin (J) , ββ1 (K), total SMAD2/3 (L) , phosphorylated SMAD2 (M) , and phosphorylated SMAD3 (N) in CRC cells with or without tricellulin overexpression and treated with SB525334 or vehicle. Representative images (O) and quantified results of Western blot analysis demonstrating protein levels of tricellulin (P) , TGFβ1 (Q) , total SMAD2/3 (R) , phosphorylated SMAD2 (S) , and phosphorylated SMAD3 (T) in CRC cells with or without tricellulin depletion treated with TGFβ1 or vehicle. n=3, * P<0.05, **P <0.01, *** P<0.001, ****P<0.0001. .

Article Snippet: To activate or inhibit the TGFβ1/SMAD pathway, cells were treated with an agonist (TGFβ1, 10 ng/ml) or antagonist (SB525334, 5 μM) (MedChemExpress, USA).

Techniques: Quantitative RT-PCR, Expressing, Over Expression, Knockdown, Western Blot

Journal: Frontiers in Oncology

Article Title: Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway

doi: 10.3389/fonc.2025.1562976

Figure Lengend Snippet: Tricellulin promotes CRC growth in vivo . Images of xenograft tumors from mice inoculated with HCT116 cells under indicated modulations (A-C) . Quantification of final tumor weights from indicated groups (D) . Tumor growth curves showing tumor volumes after cell implantation. Representative images of IHC staining of tricellulin, TGFβ1, SMAD2/3, and Ki67 in xenograft tumors (F) . Quantified expression levels of IHC staining intensity for tricellulin (G) , TGFβ1 (H) , SMAD2/3 (I) , and Ki67 (J) . Scale bar = 20 μm. n=5, * P<0.05, **P <0.01.

Article Snippet: To activate or inhibit the TGFβ1/SMAD pathway, cells were treated with an agonist (TGFβ1, 10 ng/ml) or antagonist (SB525334, 5 μM) (MedChemExpress, USA).

Techniques: In Vivo, Immunohistochemistry, Expressing